ITGA5 & ITGB6 Heterodimer Protein|使用说明书|技术资料

ITGA5 & ITGB6 Heterodimer蛋白产品信息

别名: ITGA5 & ITGB6
表达方式: 
A DNA sequence encoding the extracellular domain (Met1-Val992) of human ITGAV (P06756-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asn707) of human ITGB6 (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human ITGAV&ITGB6 heterodimer was purified.
种属: Human
表达宿主: Human Cells

ITGA5 & ITGB6 Heterodimer Protein QC Testing

纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Phe 31 & Gly 22
分子量: 
The recombinant heterodimer of human ITGAV&ITGB6 comprises 1731 (1003+728) amino acids and has a calculated molecular mass of 190.4 (111.1+79.3) KDa. As a result of glycosylation, the apparent molecular mass of human ITGAV&ITGB6 heterodimer is approximately 119 and 89-105 KDa in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

ITGA5 & ITGB6 Heterodimer Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ITGA5 & ITGB6 Heterodimer蛋白背景综述

Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

参考文献

Sosnoski DM. et al., 1988, J Clin Invest. 81 (6): 1993-8.
Tani T T. et al., 2001, J Biol Chem. 276 (39): 36535-42.
Krissansen GW. et al., 1992, Immunogenetics. 35 (1): 58-61.

规格：10ug

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