TNFRSF1B / TNFR2 / CD120b蛋白产品信息
别名: TNFRSF1B, TNFBR, TNFR2
表达方式:
A DNA sequence encoding the human TNFRSF1B (P20333-2)(Met1-Arg268, natural variant 196Met/Arg) was expressed with a C-terminal polyhistidine tag.
种属: Human
表达宿主: Human Cells
TNFRSF1B / TNFR2 / CD120b Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
生物活性:
Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 5-40 ng/mL in the presence of 1 ng/mL recombinant human TNF-α.
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Leu 23
分子量: The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
TNFRSF1B / TNFR2 / CD120b Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNFRSF1B / TNFR2 / CD120b蛋白背景综述
Tumor necrosis factor receptor superfamily member 1B, also known as TNFRII, TNFRSF1B, and CD120b, is a member to the TNFR (tumor necrosis factor receptor) superfamily characterized by cysteine-rich extracellular domains. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage–pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.
参考文献
Philippe D. et al., 2002, Arthritis Care & Research. 46 (8): 2039-44.
Wassink T H. et al., 2000, Molecular psychiatry. 5 (6): 678-82.
Alicia SC. et al., 2005, Molecular and Cellular Biology. 25 (11): 4716-26.
Ziegenhagen MW. et al., 2000, Journal of Internal Medicine. 248 (1): 33-41.
Iris Shai. et al., 2005, Diabetes Care. 28 (6): 1376-82.
规格：50ug
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