GPHA2 / Glycoprotein hormone alpha 2 Protein|技术资料|价格

GPHA2 / Glycoprotein hormone alpha 2蛋白产品信息

别名: GPHA2, GPA2, ZSIG51
表达方式: 
A DNA sequence encoding the human GPHA2 (Q96T91) (Met1-Tyr129) was  fused with Fc region of mouse IgG at the C-terminus.
种属: Human
表达宿主: Human Cells

GPHA2 / Glycoprotein hormone alpha 2 Protein QC Testing

纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Gln 24
分子量: 
The recombinant human GPHA2/mFc is a disulfide-linked homodimer. The reduced monomer comprises 340 amino acids and has a predicted molecular mass of 38.1 kDa. The apparent molecular mass of the protein is approximately 38-43 in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

GPHA2 / Glycoprotein hormone alpha 2 Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GPHA2 / Glycoprotein hormone alpha 2蛋白背景综述

GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.

参考文献

Hsu SY. et al., 2002, Science. 295 (5555): 671-4.
Suzuki C. et al., 2007, Regul Pept. 142 (1-2): 60-7.
Breous E. et al., 2006, Mol Cell Endocrinol. 245 (1-2): 169-80.

规格：20ug

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