Rat PDGFRa / CD140a Protein|鼠PDGFRa / CD140a蛋白质|使用说明|厂家现货

PDGFRa / CD140a蛋白产品信息

别名: PDGFRA
表达方式: 
A DNA sequence encoding the rat PDGFRA (G3V6A0) (Met1-Glu523) was expressed with the Fc region of human IgG1 at the C-terminus.
种属: Rat
表达宿主: Human Cells

PDGFRa / CD140a Protein QC Testing

纯度: (96.6+2.8) % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Leu 24
分子量: 
The recombinant rat PDGFRA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 741 amino acids and has a predicted molecular mass of 83.2 kDa. The apparent molecular mass of the protein is approximately 112-120 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

PDGFRa / CD140a Protein Usage Guide

储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PDGFRa / CD140a蛋白背景综述

PDGFRα, also known as CD140a, together with the structurally homolog protein PDGFRβ (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin -like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRα is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRβ dose not bind PDGF-AA. PDGF binding induces the receptor homo- and hetero-dimerization as well as activation of the receptor, and subsequently activates c-Src tyrosine kinase which relays signals to PI 3 kinase and MAPK cascades. PDGFRα thus plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGF Rα acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development.

参考文献

Matsui T. et al., 1989, Science. 243: 800-4.
Yokote K. et al., 1996, J Biol Chem. 271: 5101-511.
Choudhury GG. et al., 2000, Kidney Int. 57: 908-17.
Brennan J. et al., 2003, Genes & Dev. 17: 800-10.
Choudhury GG. et al., 2006, Cell Signal. 18: 1854-64.

规格：50ug

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