IFNAR1 / IFNAR蛋白产品信息
别名: IFNAR1
表达方式:
A DNA sequence encoding the cynomolgus IFNAR1 (NP_001253442.1) (Met1-Lys437) was expressed with six amino acids (LEVLFQ) at the C- terminus.
种属: Cynomolgus
表达宿主: Human Cells
IFNAR1 / IFNAR Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 25
分子量:
The recombinant cynomolgus IFNAR1 is a disulfide-linked homodimer. The reduced monomer comprises 420 amino acids and has a calculated molecular mass of 48.3 KDa.The apparent molecular mass of the protein is approximately 67-77 KDa in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IFNAR1 / IFNAR Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IFNAR1 / IFNAR蛋白背景综述
Interferon alpha/beta receptor 1, also known as IFN-alpha/beta receptor 1, Type I interferon receptor 1, Cytokine receptor family 2 member 1, Cytokine receptor class-II member 1, CRF2-1, IFNAR1 and IFNAR, is a single-pass type I membrane protein which belongs to the type I I cytokine receptor family. IFNAR1 is a receptor for interferons alpha and beta which contains three fibronectin type-III domains. The binding of IFNAR1 to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Ubiquitination, endocytosis, and lysosomal degradation of the IFNAR1 subunit of the type I interferon (IFN) receptor is mediated by the SCFbeta-Trcp (Skp1-Cullin1-F-box protein beta transducin repeat-containing protein) E3 ubiquitin ligase in a phosphorylation-dependent manner. Stability of IFNAR1 is regulated by its binding to Tyk2 kinase. Expression of Tyk2 stabilized IFNAR1 in a manner that was dependent neither on its binding to beta-Trcp nor IFNAR1 ubiquitination.
参考文献
Abramovich C. et al., 1994, FEBS Lett. 338: 295-300.
Colamonici O. et al., 1994, Mol Cell Biol. 14: 8133-42.
Kumar KG. et al., 2004, J Biol Chem. 279 (45): 46614-20.
Gauci S. et al., 2009, Anal Chem. 81: 4493-501.
Wollscheid B., et al., 2009, Nat Biotechnol. 27: 378-86.
Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.
规格：50ug
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