Neuropilin-1 / NRP1蛋白产品信息
别名: NRP1
表达方式:
A DNA sequence encoding the cynomolgus NRP1 (G7PEQ1) (Met1-Gly613) was expressed with six amino acids (LEVLFQ) at the C- terminus.
种属: Cynomolgus
表达宿主: Human Cells
Neuropilin-1 / NRP1 Protein QC Testing
纯度: > 85 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Ala 25
分子量:
The recombinant cynomolgus NRP1 is a disulfide-linked homodimer. The reduced monomer comprises 599 amino acids and has a calculated molecular mass of 67.5 KDa.The apparent molecular mass of the protein is approximately 67-77 KDa in SDS-PAGE.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
Neuropilin-1 / NRP1 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Neuropilin-1 / NRP1蛋白背景综述
Neuropilin-1 (Npn-1, NRP1) is a type I transmembrane protein mainly expressed in endothelial cells and tumor cells. The extracellular domain of NRP1contains two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. It can form homo- and hetero-oligomers via their MAM domains. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. NRP1 is a receptor for two unrelated ligands with disparate activities, vascular endothelial growth factor-165 (VEGF 165), an angiogenesis factor, and semaphorin/collapsins, mediators of neuronal guidance. In addition, NRP1 also binds placenta growth factor-2 and Heparin. As a co-receptor with VEGFR-2 on the surface of endothelial cells (EC), NRP1 enhances VEGF165 binding to VEGFR-2 and EC chemotaxis. Overexpression of NRP1 promotes growth and angiogenesis of tumor, as well as proliferation and chemotaxis of leukemic cells. Recent study has revealed that neuropilin-1 is also expressed by dentritic cells (DC) and resting T cells, and plays a role in initiation of the primary immune response.
参考文献
He Z. et al., 1997, Cell. 90: 739-51.
Miao HQ. et al., 1999, J Cell Biol. 146: 233-42.
Soker S. et al., 1998, Cell. 92: 735-45.
Schuch G. et al., 2002, Blood. 100: 4622-8.
Mamluk R. et al., 2002, J Biol Chem. 277: 24818-25.
Soker S. et al., 2002, J Cell Biochem. 85: 357-68.
Tordjman R. et al., 2002, Nat Immunol. 3: 477-82.
规格：20ug
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