S100A4蛋白产品信息
别名: S100A4, Capl, Mts1
表达方式:
A DNA sequence encoding the mouse S100A4 (P07091) (Ala2-Lys101) was expressed with the Fc region of human IgG1 at the N-terminus.
种属: Mouse
表达宿主: Human Cells
S100A4 Protein QC Testing
纯度: > 95 % as determined by SDS-PAGE SDS-PAGE
内**: < 1.0 EU per μg of the protein as determined by the LAL method
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu
分子量:
The recombinant mouse S100A4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 360 amino acids and has a predicted molecular mass of 40 kDa. The apparent molecular mass of the protein is approximately 38-42 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
S100A4 Protein Usage Guide
储存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
S100A4蛋白背景综述
S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
参考文献
Ambartsumian N. et al., 1995, Gene. 159: 125-30.
Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22.
Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8.
Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30.
Boye K. et al., 2008, Int J Cancer. 123: 1301-10.
Garrett SC. et al., 2006, J Biol Chem. 281: 677-80.
Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
规格：50ug
关 键 词：Mouse S100A4 Protein,使用说明,厂家现货，实验室耗材，生化试剂，上海创赛，博飞美科，实验仪器，厂家
CANSPEC品质提供的Mouse S100A4 Protein|使用说明|厂家现货性能**，其优良的品质满足您不同实验的多种需求。
本产品由上海创赛品质提供，欢迎咨询国内免费服务专线：400-608-7598，更多产品信息请登录www.canspecsci.com 或 www.4006087598.com
