CPLX3 / Complexin 3 Protein|厂家|CPLX3 / Complexin 3蛋白|技术资料

CPLX3 / Complexin 3 Protein Product Information

Synonym : CPLX3
Protein Construction: A DNA sequence encoding the human CPLX3 (Q8WVH0) (Met1-Lys154) was expressed with an N-terminal polyhistidine tag.
Source: Human
Expression Host: Human Cell

CPLX3 / Complexin 3 Protein QC Testing

Purity: > 95 % as determined by SDS-PAGE SDS-PAGE
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: His
Molecular Mass: The recombinant human CPLX3 comprises 174 amino acids and has a predicted molecular mass of 19.5 kDa. The apparent molecular mass of the protein is approximately 29-32 kDa in SDS-PAGE under reducing conditions.
Formulation: Lyophilized from sterile PBS, pH 7.4.
                 1.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
                 2.Please contact us for any concerns or special requirements.

CPLX3 / Complexin 3 Protein Usage Guide

Storage:  Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:  A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CPLX3 / Complexin 3 Protein Description

CPLX3, also known as complexin 3, belongs to the complexin/synaphin family. As a SNARE-binding protein, complexin (CPX), can act either as a facilitator or as an inhibitor of membrane fusion, constituting a controversial dilemma. CPX acts sequentially on assembling SNAREpins, first facilitating zippering by nearly doubling the distance at which v- and t-SNAREs can engage and then clamping them into a half-zippered fusion-incompetent state. Specifically, the central helix of CPX allows SNAREs to form this intermediate energetic state at 9-15 nm but not when the bilayers are closer than 9 nm. Stabilizing the activated-clamped state at separations of less than 9 nm requires the accessory helix of CPX, which prevents membrane-proximal assembly of SNAREpins. CPLX3 binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.

References

Newton-Cheh C. et al., 2009, Nat Genet. 41(6): 666-76.
Li F. et al., 2011, Nat Struct Mol Biol. 18 (8): 941-6.
Amin N. et al., 2012, Mol Psychiatry. 17 (11): 1116-29.

规格：50ug

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