C22-125-06 rMuVEGF120说明书

Catalog Number:  C22-125-06
Source:  Escherichia coli.
Molecular Weight:  Recombinant murine VEGF120 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two
 121 amino acid polypeptide chains.
Quantity:  2μg/10μg/500μg
AA Sequence:  MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC
 CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
Purity:  >96% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay using human umbilical vein endothelial cells(HUVEC) is less than 4 ng/ml, corresponding to a specific activity of＞ 2.5 × 105 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rMuVEGF120 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor (VEGF) was initially purified from media conditioned by normal bovine pituitary folliculo-
stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently
found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in
media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse
cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or
188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse
homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in
their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the
heterodimer ofKDR/Flt in VEGF signal transduction remain to be elucidated.In vivo, VEGF has been found to be a potent
angiogenesis inducer.