C22-125-04 rMuEGF说明书

Catalog Number:  C22-125-04
Source:  Escherichia coli.
Molecular Weight:  Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids,
 including 3 intramolecular disulfide-bonds.
Quantity:  100μg/500μg/1000μg
AA Sequence:  NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
Purity:  >97% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of＞ 1.0 × 107 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rMuEGF as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -
 70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Epidermal Growth Factor
Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse
submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of
growth when injected into newborn mice. EGF is a potent growth factor that stimulates the proliferation of various epidermal and
epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals
through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and
VGF (vaccinia virus growth factor).