C22-103-07 rHuLIF说明书

Catalog Number:  C22-103-07
Source:  Escherichia coli.
Molecular Weight:  Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity:  5μg/10μg/1000μg
AA Sequence:  SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE
 PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD
 QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT
 SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Purity:  >98% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The specific activity determined by inducing
 differentiation of murine M1 myeloid leukemic cells. The minmun detectable concentration of rHuLIF in this assay is 0.5 ng/ml, corresponding to a specific activity of＞2×106 IU/mg, where 50 units is defined as the amount of rHuLIF required to induce differentiation in 50% of the M1 colonies in 1ml agarcultures.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH 7.4, with 0.02% TWEEN  20.
Endotoxin:  Less than 1EU/μg of rHuLIF as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Human Leukemia inhibitory factor
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by
suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control
of stem cell pluripotency,bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of
megakaryocyte production in vivo.