C22-106-05 rHuIFN-β1b说明书

Catalog Number:  C22-106-05
Source:  Escherichia coli.
Molecular Weight:  Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 169 amino acids.
Quantity:  2μg/10μg/1000μg
AA Sequence:  MSYNLLGFLQ RSSNFQSQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY
 EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL
 HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
Purity:  >96% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The specific activity determined by an anti-viral
 assay is no less than 4.0 × 107 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, containing 3% manitol.
Endotoxin:  Less than 1EU/μg of rHuIFN-β1b as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further
 evaluation purposes. NOT FOR HUMAN USE.

Human Interferon-β
Interferon-beta is one of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by
double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-
beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is
induced also by some cytokines such as TNF and IL-1.