C22-166-07 rOvIFN-τ说明书

Catalog Number: C22-166-07
Source:  Pichia. Pastoris.
Molecular Weight:  Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 172 amino acids.
Quantity:  2μg/10μg/1000μg
AA Sequence:  CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ EMVEGDQLQK
 DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT LLEQLCTGLQ QQLDHLDTCR
 GQVMGEEDSE LGNMDPIVTV KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR
 ALTVSTTLQK RLTKMGGDLN SP
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral
 resistance assay is no less than 1.0 × 107 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rOvIFN-tau as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further
 evaluation purposes. NOT FOR HUMAN USE.

Ovine Interferon-tau
IFN-τis a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in
sheep. IFN-τhas potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-α/
β. The current investigation concerns the effect of recombinant ovine IFN-tau (rOvIFN-τ) on the modulation of MHC class I
and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1
and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS
is via interference with IFN-γ-induced MHC class II expression. IFN-τwas shown to downregulate IFN-γ-induced MHC
class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central
nervous system (CNS). IFN-τdid not upregulate the expression of MHC class II on CVE. IFN-τalso inhibited the replication of
Theiler's virus in CVE.