C22-121-01B rMuIL-1β说明书

Catalog Number:  C22-121-01B
Source:  Escherichia coli.
Molecular Weight:  Approximately 17.5kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity:  2μg/10μg/1000μg
AA Sequence:  MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA
 LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN
 WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS
Purity:  >98% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay usingmurine D10S cells is less than 0.002 ng/ml, corresponding to a specific activity of＞ 5.0
 × 108 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rMuIL-1β as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Interleukin-1 beta
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages,
keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical
biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by
inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of
prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-
associated cytokine.