C22-108-02 rHuBMP-2说明书

Catalog Number:  C22-108-02
Source:  Escherichia coli.
Molecular Weight:  Approximately 26 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated
 polypeptide chains.
Quantity:  2μg/10μg/1000μg
AA Sequence:  MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPF PLADHLNSTN
 HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by inducing alkaline phosphatase production ofmurine ATDC5 cells is less than 200 ng/ml, correspond-ing to a specific activity of ＞ 5.0 × 103 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution containing 10mM sodium citrate pH 3.5.
Endotoxin:  Less than 1EU/μg of rHuBMP-2 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Human Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins (BMPs) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a
potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such
as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac
morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The
functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single
disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23
amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds
between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type
protease.