Human Kallikrein 3/PSA Biotinylated Affinity Purified PAb

产品名称：Human Kallikrein 3/PSA Biotinylated Affinity Purified PAb

产品规格：50UG

单        位：支

关 键 字：anti-human PSA,uman Kallikrein 3/PSA Biotinylated Affinity Purified PAb,ELISA,WB

CANSPEC品质提供的Human Kallikrein 3/PSA Biotinylated Affinity Purified PAb性能**，中文名称为Human Kallikrein 3/PSA。

产品Human Kallikrein 3/PSA Biotinylated Affinity Purified PAb以其优良的品质满足您不同实验的多种需求。并保证每批Human Kallikrein 3/PSA Biotinylated Affinity Purified PAb的质量和品质优良。

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ORDERING INFORMATION    Produced in goats immunized with purified, NS0-derived, recombinant human Kallikrein 3 (rhKLK3; aa 18 - 261). Human KLK3 specific IgG was purified by human         KLK3 affinity chromatography and then biotinylated.
Catalog Number: BAF1344
Lot Number: IRI03
Size: 50 μg
Formulation  Lyophilized from a 0.2 μm filtered solution in phosphate-buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of antibody.
Formulation: 0.2 μm filtered solution in PBS  with BSA
Storage: -20° C
Reconstitution   Reconstitute with sterile Tris-buffered saline pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% BSA. If 1 mL of buffer is used, the antibody concentration will be 50 μg/mL.
Reconstitution: sterile 0.1% BSA in TBS
Specificity: human KLK3
Immunogen: NS0-derived rhKLK3
Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Ig Type: goat IgG
Applications: Western blot ELISA detection
Specificity
This antibody has been selected for use as a detection antibody in human KLK3 ELISAs and Western blots.
Applications
Western blot - This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human KLK3. The detection limit for rhKLK3 is approximately 20 ng/lane under non-reducing and reducing conditions.
ELISA detection - This biotinylated antibody can be used as a detection reagent in a human KLK3 sandwich immunoassay in combination with the human KLK3 capture reagent (Cat. # MAB13442) and recombinant human KLK3 (Cat. # 1344-SE) as the standard. The suggested concentration range for this detection reagent is 0.1 - 0.4 μg/mL and should be titrated to determine the optimal concentration. A general protocol is provided at www.RnDSystems.com/MAPELISA. In this format, less than 0.5% cross-reactivity is observed with rhKLK-1 and rhKLK-2.
Optimal dilutions should be determined by each laboratory for each application.