FITC Rat Anti-Mouse Ly-6A/E

产品名称:FITC 大鼠抗小鼠 Ly-6A/E 抗体；FITC Rat Anti-Mouse Ly-6A/E

中文名称:FITC 大鼠抗小鼠 Ly-6A/E 抗体

英文名称:FITC Rat Anti-Mouse Ly-6A/E

FITC Rat Anti-Mouse Ly-6A/E 产品介绍：

Material Number: 557405
Alternate Name: Sca-1
Size: 0.1 mg
Concentration: 0.5 mg/ml
Clone: D7
Immunogen: IL-2-dependent mouse T-cell line CTL-L
Isotype: Rat (LEW) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide



FITC Rat Anti-Mouse Ly-6A/E Description：

The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a 
phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of 
mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 
129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T 
lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral 
lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies 
with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to 
be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of 
FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated 
E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 
559971) to identify HSC.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.