APC Rat Anti-Mouse CD25

产品名称:APC 大鼠抗小鼠 CD25 抗体；APC Rat Anti-Mouse CD25

中文名称:APC 大鼠抗小鼠 CD25 抗体

英文名称:APC Rat Anti-Mouse CD25

APC Rat Anti-Mouse CD25 产品介绍：

Material Number: 557192
Alternate Name: Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: PC61
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Isotype: Rat (OFA) IgG1, λ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.


APC Rat Anti-Mouse CD25 Description：

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from
all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132)
chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not
express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low
TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg-
Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of
T-cell development in fetal and ***** thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the
maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The
PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No.
557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.