APO-BRDU™ Kit

产品名称:APO-BRDU™ Kit

APO-BRDU™ Kit 产品介绍：

Material Number: 556405
Size: 60 Tests
Component: 51-6576AK
Description: APO-BRDU™ Kit Part A
Component: 51-6576BK
Description: APO-BRDU™ Kit Part B


APO-BRDU™ Kit Description：

One of the later steps in apoptosis is DNA fragmentation, a process which results from the activation of endonucleases during the apoptotic
program. These nucleases degrade the higher order chromatin structure into fragments of ~300 kb and subsequently into smaller DNA pieces
of about 50 bp length. A method which is often used to detect fragmented DNA utilizes a reaction catalyzed by exogenous TdT, often referred
to as "end-labeling" or "TUNEL" (terminal deoxynucleotidyltransferase dUTP nick end labeling). In the APO-BRDU™ assay, TdT catalyzes a
template-independent addition of bromolated deoxyuridine triphosphates (Br-dUTP) to the 3'-hydroxyl (OH) termini of double- and
single-stranded DNA. After incorporation, these sites are identified by flow cytometric means by staining the cells with a FITC-labeled
anti-BrdU mAb. The APO-DIRECT™ assay (Cat. No. 51-6536KK) is a single-step method for labeling DNA breaks with FITC-dUTP,
followed by flow cytometric analysis.

1.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.