APO-DIRECT™ Kit

产品名称:APO-DIRECT™ Kit

APO-DIRECT™ Kit 产品介绍：

Material Number: 556381
Size: 50 Tests
Component: 51-6536AK
Description: APO-DIRECT™ Kit Part A
Component: 51-6536BK
Description: APO-DIRECT™ Kit Part B

APO-DIRECT™ Kit Description：

One of the later steps in apoptosis is DNA fragmentation, a process which results from the activation of endonucleases during the apoptotic
program. These nucleases degrade the higher order chromatin structure into fragments of ~300 kb and subsequently into smaller DNA pieces
of about 50 bp in length. A method which is often used to detect fragmented DNA utilizes a reaction catalyzed by exogenous TdT, often
referred to as "end-labeling" or "TUNEL" (terminal deoxynucleotidyltransferase dUTP nick end labeling). The APO-DIRECT™ assay (Cat.
No. 6536KK) is a single-step method for labeling DNA breaks with FITC-dUTP, followed by flow cytometric analysis. In the APO-BRDU™
assay, TdT catalyzes a template-independent addition of bromolated deoxyuridine triphosphates (Br-dUTP) to the 3'-hydroxyl (OH) termini of
double- and single-stranded DNA. After incorporation, these sites are identified by flow cytometric means by staining the cells with a
FITC-labeled anti-BrdU mAb.

1.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.