APC Rat Anti-Mouse CD62L

产品名称:APC 大鼠抗小鼠 CD62L 抗体；APC Rat Anti-Mouse CD62L

中文名称:APC 大鼠抗小鼠 CD62L 抗体

英文名称:APC Rat Anti-Mouse CD62L

APC Rat Anti-Mouse CD62L 产品介绍：

Material Number: 553152
Alternate Name: L-selectin, LECAM-1, Ly-22
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: MEL-14
Immunogen: C3H/eb mouse B lymphoma 38C-13
Isotype: Rat (F344) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.



APC Rat Anti-Mouse CD62L Description：

The MEL-14 antibody reacts with CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and 
Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an 
immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T 
lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for 
lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed 
from lymphocytes and neutrophils upon cell activation, metalloproteinases may mediate the release of CD62L ectodomains from the cell 
surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to 
sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that 
CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may 
be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte 
extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

APC Rat Anti-Mouse CD62L Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.