FITC Rat Anti-Mouse CD62L

产品名称:FITC 大鼠抗小鼠 CD62L 抗体；FITC Rat Anti-Mouse CD62L

中文名称:FITC 大鼠抗小鼠 CD62L 抗体

英文名称:FITC Rat Anti-Mouse CD62L

FITC Rat Anti-Mouse CD62L 产品介绍：

Material Number: 553150
Alternate Name: L-selectin, LECAM-1, Ly-22
Size: 0.5 mg
Concentration: 0.5 mg/ml
Clone: MEL-14
Immunogen: C3H/eb mouse B lymphoma 38C-13
Isotype: Rat (F344) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.




FITC Rat Anti-Mouse CD62L Description：

The MEL-14 antibody reacts with CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and 
Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an 
immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T 
lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for 
lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from 
lymphocytes and neutrophils upon cell activation, metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. 
The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed 
oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, 
GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be ligands 
for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte 
extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

FITC Rat Anti-Mouse CD62L Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.