APC Rat Anti-Mouse CD45R/B220

产品名称:APC 大鼠抗小鼠 CD45R/B220 抗体；APC Rat Anti-Mouse CD45R/B220

中文名称:APC 大鼠抗小鼠 CD45R/B220 抗体

英文名称:APC Rat Anti-Mouse CD45R/B220

APC Rat Anti-Mouse CD45R/B220 产品介绍：

Material Number: 553092
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: RA3-6B2
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Isotype: Rat IgG2a, κ
Reactivity: QC Testing: Mouse
Tested in Development: Human
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.



APC Rat Anti-Mouse CD45R/B220 Description：

The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the 
transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B 
lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. 
The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T 
cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK 
cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell 
precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been 
reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine 
Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly 
variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The 
CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell 
and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker; however, CD19 expression, detectable by 
the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody 
(clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. 
Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.

APC Rat Anti-Mouse CD45R/B220 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.