PE Rat anti-Mouse CD34

产品名称:PE 大鼠抗小鼠 CD34 抗体；PE Rat anti-Mouse CD34

中文名称:PE 大鼠抗小鼠 CD34 抗体

英文名称:PE Rat anti-Mouse CD34

PE Rat anti-Mouse CD34 产品介绍：

Material Number: 551387
Size: 0.2 mg
Concentration: 0.2 mg/ml
Clone: RAM34
Immunogen: Recombinant Mouse CD34
Isotype: Rat IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium 
azide.


PE Rat anti-Mouse CD34 Description：

The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. 
RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for 
expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are 
also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 
expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low 
Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small 
percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in 
this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, 
and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E 
(Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally 
irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the 
bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is 
CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-. 

PE Rat anti-Mouse CD34 Preparation and Storage：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.