产品名称:APC 小鼠抗人 IFN-γ 抗体;APC Mouse Anti-Human IFN-γ
中文名称:APC 小鼠抗人 IFN-γ 抗体
英文名称:APC Mouse Anti-Human IFN-γ
APC Mouse Anti-Human IFN-γ 产品介绍:
Material Number: 551385 Size: 50 tests Vol. per Test: 20 µl Clone: 4S.B3 Immunogen: Partially purified human IFN-γ from supernatants of human PBMC stimulated with Staphylococcus aureus Isotype: Mouse IgG1, κ Reactivity: Human QC Testing: Baboon or Rhesus or Cynomolgus Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide APC Mouse Anti-Human IFN-γ Description:
Clone 4S.B3 reacts with the human form of interferon-γ (IFN-γ) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ+ cells within a mixed cell population.
APC Mouse Anti-Human IFN-γ Preparation and Storage:
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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