PE Active Caspase-3 Apoptosis Kit

产品名称:PE Active Caspase-3 Apoptosis Kit

PE Active Caspase-3 Apoptosis Kit 产品介绍：

Material Number: 550914
Reactivity: QC Testing: Human
Tested in Development: Mouse
Component: 51-68655X
Description: PE Rabbit Anti- Active Caspase-3 (CPP32; Yama; Apopain)
Size: 100 Tests (1 ea)
Vol. per Test: 20 µl
Clone Name: C92-605
Immunogen: Human Active Caspase-3 Fragment
Isotype: Rabbit IgG
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Component: 51-6896KC
Description: Cytofix/Cytoperm™ Fixation and Permeabilization Solution (1X)
Size: 65 ml (1 ea)
Storage Buffer: Aqueous buffered solution containing paraformaldehyde and saponin.
Component: 51-6897KC
Description: Perm/Wash™ Buffer (10X Solution)
Size: 65 ml (1 ea)
Storage Buffer: Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09% 
sodium azide.

The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during 
the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells 
undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) 
and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists 
of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and 
activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody 
has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the 
pro-enzyme form of caspase-3.

PE Active Caspase-3 Apoptosis Kit Preparation and Storage：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

PE Active Caspase-3 Apoptosis Kit Product Notices：

 1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental 
sample (a test).