DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion Protein

产品名称:DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion  Protein

DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion  Protein 产品介绍：

Material Number: 550750
Size: 0.25 mg
Concentration: 0.5 mg/ml
Isotype: Mouse IgG1, λ
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.


DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion  Protein Description：

The H-2K[b]:Ig fusion protein consists of three extracellular major histocompatibility complex (MHC) class I H-2Kb domains that are fused to the VH regions of mouse IgG1. In order for the MHC class I to be functional, i.e., capable of binding peptides, β2 Microglobulin (β2M) must be present. For this reason, BD™ DimerX consists of recombinant H-2K[b]:Ig fusion protein, supplemented with recombinant β2M. Recombinant MHC molecules, like the BD DimerX fusion protein, are useful for studying T-cell function by immunofluorescent staining and flow cytometric analysis of antigen-specific T cells.

DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion  Protein Preparation and Storage：

Store undiluted at 4°C.
The H-2K[b] protein was expressed together with human β2M in the mouse plasmacytoma cell line, J558L (ATCC TIB-6). The 
H-2K[b] and β2 polypeptide chains are associated noncovalently as a consequence of their coexpression within J558L cells. 
The H-2K[b]:Ig fusion protein was purified from tissue culture supernatant by affinity chromatography. The purity of the 
preparation was confirmed by SDS-PAGE.

DimerX I: Recombinant Soluble Dimeric Mouse H-2K[b]:Ig Fusion  Protein Suggested Companion Products：

Catalog Number Name Size Clone
550083 PE Rat Anti-Mouse IgG1 0.1 mg A85-1
553485 Purified Mouse IgG1 λ Isotype Control 0.5 mg A111-3
551089 Recombinant Human β2 Microglobulin 0.1 mg (none)
554656 Stain Buffer (FBS) 500 ml (none)
553141 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 0.1 mg 2.4G2