产品资料

无细胞蛋白表达Expressway系列

产品名称: 无细胞蛋白表达Expressway系列
产品型号: K990097
产品展商: GIBCO
产品文档: 无相关文档

简单介绍

能够在两小时内得到高产量高质量的蛋白产品 线状或换装的DNA模板都可以工作 便捷的单管操作，便于放大表达以及高通量表达 先进的克隆及检测技术，方便下游的结构功能研究


无细胞蛋白表达Expressway系列  的详细介绍
详细说明：
The Expressway™ Maxi Cell-Free E. coli Expression System uses an efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4-6 hours. The procedure can be performed in a single reaction tube and is easily scalable without the need for specialized equipment. The TOPO TA Cloning® expression vectors (5-min cloning with 95% efficiency) are provided for optimal expression results. In addition to all the advantages of an open expression system, Expressway™ Maxi technology provides a means to produce high levels of recombinant protein that may be easily detected and purified for various downstream applications.

Milligram level protein production within 4-6 hours
The key to the Expressway™ Maxi technology is the unique formulation of the Feed Buffer that boosts the reaction productivity so that more than one milligram of protein is produced in a 2-ml reaction within 4-6 hours

常用规格

Expression Mechanism:

Cell-Free Expression 
Expressed Protein Type:

Water Soluble Protein 
Brand:

Expressway™ 
Expression:

Cell-Free 
Product Size:

1 kit 

内容和储存

The Expressway™ Maxi Cell-Free Expression System is designed to perform two hundred 25-µl, five 1-ml, or one 5-ml (initial volume) reactions or one to several large reactions, depending on your needs. This system includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway™ Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO® and pEXP5-CT/TOPO® also includes the pEXP5-NT/TOPO® and pEXP5-CT/TOPO® TA Expression Kits. Each kit contains the pEXP5-NT/TOPO® or pEXP5-CT/TOPO® vector, other reagents to facilitate TOPO® Cloning, and One Shot® TOP10 Chemically Competent E. coli. Store the vectors at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.

相关信息：

产品

规格

货号

Expressway™ Maxi Cell-Free E. coliExpression System

1kit

K990097

Expressway™ Mini Cell-Free Expression System

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K990100

Expressway™ Tag-On-Demand™ Cell-Free E. coli Expression System

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Expressway™ N-term Lumio™ Expression and Detection System

1kit

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Expressway™ Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO® and pEXP5-CT/TOPO® vectors

1kit

K990096

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