产品资料

细胞解离产品

产品名称: 细胞解离产品
产品型号: C14-13150-016
产品展商: GIBCO
产品文档: 无相关文档

简单介绍

细胞解离液Cell dissociation buffer 不含酶,由Hank’s液配制 保存:15-30°


细胞解离产品  的详细介绍
详细说明:
Cell Dissociation Buffers are membrane-filtered, isotonic, and enzyme-free aqueous formulations of salts, chelating agents, and cell-conditioning agents in either Ca2+- and Mg2+-free Hanks balanced salt solution or Ca2+- and Mg2+-free phosphate-buffered saline. They are suitable for gentle dissociation of mammalian cells from support substrates and each other, for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry (1,2). These buffers are not recommended for routine passage of strongly adherent cell types.

Performance and quality testing: Buffers are membrane filtered and mycoplasma tested. Cell integrity after dissociation with buffers is measured with a plating efficiency assay and a serotonin receptor function assay.

常用规格

Chelators:

EDTA 

Product Size:

100 ml 

Classification:

Animal Origin-Free 

Enzyme Stability:

Room Temperature Stable 

Phenol Red Indicator:

No Phenol Red 


相关信息:

品名

产品描述

货号

规格

细胞解离液

Cell dissociation buffer

不含酶,由Hank’s液配制

保存:15-30°

13150-016

100ml

细胞解离液

Cell dissociation buffer

不含酶,由PBS配制

保存:15-30°

13151-014

100ml

胶原酶,collagenase

活性:每毫克不少于125U2-8°

17018-029

500mg

I型胶原酶,collagenase Type I

可解离组织,建议用于上皮、肺、脂肪和肾上腺组织细胞,2-8°保存

17100-017

1g

II型胶原酶,collagenase Type II

可解离组织,建议用于肝、骨、甲状腺、心和唾液腺组织细胞,2-8°保存

17101-015

1g

IV型胶原酶,collagenase Type IV

可解离胰腺组织,用于分离胰岛,

2-8°保存

17104-019

1g

分散酶,Dispase

温和的解离组织得到单个细胞,

2-8°保存

17105-041

5g

胰酶替代物,TrypLE Select

配方包含1mM EDTADPBS

不含酚红,保存:2-8°

12563-011

100ml

 

12563-029

500ml

胰酶替代物,TrypLE Express

配方包含1mM DPBS

含酚红,保存:2-8°

12605-010

100ml

 

12605-028

500ml

胰酶替代物,TrypLE Express

配方包含1mMDPBS

不含酚红,保存:2-8°

12604-013

100ml

 

12604-021

500ml

胰酶,Trpsin0.25%

不含钙镁离子,含酚红

保存:-5 - -20°

15050-065

100ml

 

15050-057

500ml

胰酶,Trpsin2.5%10X

无菌条件下以Hank’s10倍稀释,

不含钙镁离子,保存:-5 - -20°

15090-046

100ml

胰酶-EDTA

0.05%胰酶,EDTA4Na

不含钙镁离子,含酚红

保存:-5 - -20°

25300-054

100ml

25300-062

500ml

胰酶-EDTA,(10X

0. 5%胰酶,EDTA4Na

无菌条件下以Hank’s10倍稀释,

不含钙镁离子,保存:-5 - -20°

15400-054

100ml

胰酶-EDTA

0.25%胰酶,EDTA4Na

不含钙镁离子,含酚红

保存:-5 - -20°

25200-056

100ml

25200-072

500ml

胰酶(1250),干粉

保存:2-8°

27250-018

100g

胰酶抑制剂,大豆

0.25-0.5mg/mlPBS溶液灭活等体积胰酶,保存:-5 - -20°

17075-029

1g

EDTAVersene15000

0.2g/l EDTA.4Na磷酸缓冲盐溶液,

=0.48Mm,保存:2-8°

15040-066

100ml

Low Trypsin-High EDTA,

0.025% 胰酶, 0.75mM EDTA (1X), 溶于PBS,不含钙镁离子

SM-2004-C

100ml

Trypsin 0.25%

Hank‘s 液,不含钙镁离子

SM-2001-C

100ml

Accutase

 

SCR005

100ml

Accumax

 

SCR006

100ml

Trypsin-EDTA

0.05% Trypsin & 0.53mM EDTA,

溶于Hank‘s液,不含钙镁

SM-2002-C

100ml

Trypsin-EDTA

0.25% Trypsin & 1mM EDTA

溶于Hank‘s液,不含钙镁离子

SM-2003-C

100ml




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